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KMID : 1123920030170061500
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Korean Journal of Oriental Physiology and Pathology
2003 Volume.17 No. 6 p.1500 ~ p.1508
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Effects of Daejo-whan on the Ischemic Damage of Cerebral Neurons in Culture
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Park Se-Hong
Lee Kwang-Ro
Bai Sun-Joon
Jung Sang-Su
Kang Se-Young
Lee Sang-Gwan
Lee Seong-Geun
Yoon Ji-Won
Sung Kang-Keyng
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Abstract
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This study was performed to clarify the neurotoxic mechanism of nerve cells damage by brain ischemia. The cytotoxic effect of ischemia was determined by XTT assay, NR assay, superoxide dismutase(SOD) activity, amount of malondialdehyde(MDA), lactate dehydrogenase(LDH) activity, protein synthesis and tumor necrosis factor(TNF)-α activities after cerebral neurons derived from mouse were exposed to ischemia for 1∼30 minutes. In addition, the protective effect of extract of Daejo-whan(DJW) on ischemia-induced neurotoxicity was examined in these cultures. 1. Ischemia decreased cell number and viability by XTT assay or NR assay when cultured cerebral neurons were exposed to 95% N2/5% CO₂ for 1∼20 minutes in these cultures. 2. Ischemia decreased SOD and protein syntheses, but it increased amount of MDA and, LDH and TNF-α activities in these cultures. 3. In the neuroprotective effect of DJW extracts on cerebral neurons damaged by ischemia, DJW extracts increased SOD activity and protein synthesis. While, it decreased amount of MDA and, LDH and TNF-α activities after cerebral neurons preincubated with herb extracts. It suggests that brain ischemia has neurotoxicity on cultured mouse cerebral neurons, and the herb extract such as DJW was very effective in blocking the neurotoxicity induced by ischemia in cultured mouse cerebral neurons.
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KEYWORD
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Daejo-whan, neurotoxicity, Ischemic Damage
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